Comparative Study of Molecular Markers for Detection · Of Genetic Diversity in Rice (Oryza Sativa) With the Help of (Rapd, Issr ,ssr & Sts) Markers

Abstract

Molecular markers provide novel tools to differentiate between the various grades of Indian rice, maintain fair-trade practices and to determine its relationship with other rice groups in Oryza sativa. We have evaluated the genetic diversity and patterns of relationships among the 24 rice genotypes representative of the traditional Basmati, cross-bred Basmati and non-Basmati rice varieties using RAPD,ISSR,SSR and STS markers. All the four marker systems generated higher levels of polymorphism and could distinguish between all the 24 rice cultivars. There were significant correlations between the Jaccard coefficients obtained using the matrices of each single marker type and their combined matrix. In the course of the present study, four RAPD and SSR primers or five STS and ISSR primers are sufficient to differentiate all varieties.Markers with high PIC values are very useful for estimating relationships between varieties. Markers with low PIC values, on the other hand, can be used to analyze chromosome regions of special interest . The value of Resolving power can also be used as future reference data for selection of primers for study using same primers we used in present study. The greater the value of resolving power the more infonnation is provided by that particular pnmer.