Assessment of Genetic Diversiqt and Interrelationship Among Pigeonpea- (Cajanus Cajan) Collections Through Rapd and Issr Markers Analysis

Abstract

Genetic variation between 42 genotypes of pigeonpea was evaluated using 10 RAPD and 10 ISSR markers. Among these markers, RAPD primers generated 40 alleles, out of which 37 were polymorphic and ISSR markers produced 56 alleles, out of which 50 were polymorphic. RAPD primers detected more polymorphic loci (92.5%) than the ISSR primers (89.3%). Mean PIC for each of these marker systems (0.8618 for RAPD and 0.7131 for ISSR}, indicated better PIC value of the RAPD markers. Comparison of resolving power of two marker systems (2.1524 for RAPD and 4.481 for ISSR), showed better resolving power of the ISSR markers. Based upon Jaccard's similarity coefficient and cluster analysis using UPGMA of both RAPD and ISSR primers, pigeonpea genotypes AL-201, UPAS-120, ICP-9174, ICP-10957 & ICPL-88034 were found to be quite distinct and they can be used for their desirable characteristic in breeding programmes. Dendograms generated using RAPD and ISSR marker data were comparable with minor clustering differences, which suggests that either method, or a combination of both can be applied to expanded genetic diversity studies in Cajanus cajan and identify variation within genotypes.