Detection of Genetic Variability in Mango (Mangifera Indica L.) by RAPD Markers

Abstract

The molecular marker is a useful tool for assessing genetic variations and resolving cui ti var identities. The objective of this study was to evaluate the genetic diversity of 22 genotypes of mango (Mangifera indica L.) using the random amplified polymorphic DNA (RAPD). In RAPD Analysis ten primers generate a total of 42 alleles. Genetic diversity of 22 genotypes estimated by polymorphic infortnation content (PIC) value ranged from 0.332 to 0.669. The cluster dendrogram of RAPD revealed 2 major clusters and Cluster I was the small and included 5 genotypes while clusters II comprising 17 genotypes. Jaccard's coefficient of similarity revealed that high degree of similarity to the extent of 90 % exists between genotypes Bombay green and Neelam chaunsa and Dasheri and Gulab jamun. Whereas Alphonso keep very low level of similarity (50%) to members of other clusters. Dendrogram revealed that the genotype Ramkela was distantly located with Alphonso with similarity coefficient 0.475. Whereas M~njiri keep very low level similarity other genotypes i.e. 70 % to ffiPmbers of other clusters. Mango genotypes evaluated using RAPD in the present study is helpful to identify potential geno es which give broadness to the gertnplasm base of mango