Studies to Identify Genes for Fragrance In Rkcje (Oryza Sativa L.) Through Gene Specific Pidiviers

Abstract

Fragrance in rice is one of the most highly valued grain quality traits, which is due to the accumulation of fragrant compound 2-acetyl-l-pyrroline (2AP). Mutation in thefgr gene results in the accumulation of 2-acetyl- I -pyrroline (2AP) in rice. The present study based on evaluation of fragrance in 40 rice cultivars (aromatic and non aromatic) by using functional marker. Forty rice varieties were planted on CRC chirodi, SVPUAT, Meerut. In the present study we screened the forty rice varieties for fragrance by using 6 gene specific primers named as BADEX7-1(48l/489) bp, BADEX7-2(39l/399) bp, BADEX7-3(265/273) bp, BADEX7-4(187/l95) bp, BADEX7-5(95/103) bp, and BADEX7-6(56/64) bp. Out of forty rice genotypes, 16 genotypes shows fragrant allele for BADEX7-l primer, BADEX7-2 primer were shows in only 13 rice genotypes, BADEX7-3 primer give an amplicon in 17 rice genotypes, BADEX7-4 primer is reported in 17 rice genotypes, BADEX7-5 primer is amplified in 17 genotypes and BADEX7-6 primer shows fragment in 16 varieties. Only two primers BADEX7-4 and BADEX7-5 could discriminate all the rice varieties into fragrant and non fragrant genotypes. So we recommend the use of this simple, low-cost marker in routine genotyping for fragrance trait in large scale breeding materials and germplasm.