In Vitro Regeneration and Assessment of Genetic Fidelity Using Rapd Markers in Gladiolus (Gladiolus Hybrida)

Abstract

G~adi~lus (Gladi~lus hybrida) is a bulbous ornamental plant, have a great commercial Importance m cut flower industry all over the world as well as in lndia.The genus Gladiolusbelongs to the family Iridaceae. In India, gladiolus . is cultivated on 11660 ha area and produce 106 crore cut flowers. In vitro propagation techniques, assumes significance, especially for securing rapid multiplication of the novel cultivarsusing different explants sources and media. In present study the in vitro regeneration of gladiolus cultivar White prosperity was achieved using shoot bud of cormels as an explant. The concentration and combination of plant growth regulators. governed the regenerative capacity of explants. The cytokinin, BAP efficiently produced multiple shoots in gladiolus on B5 and .MS media. The number of shoots varied from1.3 to 3.0 shoots per explant on B5 media and 0.6 to 2.3 shoots per explant on MS media. The length of in-vitro developed multiple shoots varied from 2.2 to 3.8 em in B5 media and 1.1 to 2.9 ~min iv[S media, 30 Days After Inoculation (DAI). The maximumnumber of shoots (3.0 and 2.3) were recordedwith treatment of 1 mg/1 BAP in both B5 and MS media respectively. Maximum rooting(l.5cm) were observed at 1.0 mg/1 BAP and 2mg/l NAA in MS media. The genetic fidelity of in vitro raised multiple shoots were determinedby Randomly Amplified Polymorphic DNA (RAPD) markers. The RAPD primers produced 35 distinct bands in fourteen in vitro regenerated clones and one mother plant. The average of scorable bands was 3.5 bands per primer. Total 525 bands were generated byall primers, showed monomorphic banding patternindicating no oenomic variations occurred in in vitro raised plants. As per the RAPD markers study all the randomly selected clones were similar to the mother plant.