Isolation and Identificaiton of Microorganism from Faecal Samples in Poultry

Abstract

The purpose of this study was to isolate and identify microorganisms from chicken faeces. The complete research project was carried out between February and April in the laboratory of Division of Biological Standardisation, Indian Veterinary Research Institute. lzntnagar, Bareilly. 30 faeces samples were used in the investigation. The entire testing process was carried out in sterile conditions. By examining colony shape, gram's staining and biochemical tests. isolates and their identities were determined. E. coli colonies on MacConkey agar- showed smooth pinkish colony. Salmonella sp. colonies on Hektoen Enteric agar were smooth black centred colonies. Confmnation of E. coli isolates were done by biochemical test. The E. coli isolates showed positive test for Indole, Catalase and MR while negative test for VP, Oxidase and Citrate. The Salmonella sp showed negative for Indole, VP, oxidase and positive for MR, citrate, catalase. A total of 24 E. coli and 6 Salmonella sp were isolated from the 30 faecal samples. The 24 E. coli isolates were found positive for fimC (496 bp) and 16S rRNA (585 bp) genes.