Studies on Phytochemical Extraction and Antimicrobial Activity of Azadirachta Indica and. Withania Somnifera

Abstract

Drugs from natural sources are used for treating various human and plant disease. Present studies were carried out in Azadirachta indica and Withania somnifera leaf. These medicinal plants have antimicrobial activity against bacterial and fungal pathogen of human and plant diseases. The leaf extract of Azadirachta indica and Withania somnifera were prepared in aqueous and 50%, 40%, and 30% ethanolic, methanolic and acetone solution at 50mg/ml concentration for checking antimicrobial activity against Aspergillus niger, Fusarium moniliforme, E. coli and Pseudomonas aeruginosa. Ethanolic 50% extract of Withania somnifera was shown maximum percent growth inhibition against Aspergillus niger (61.76%) and Fusarium moniliforme (56.52%) then acetone, methanolic and aqueous extract. Azadirachta indica 50% ethanolic extracts was observed maximum zone of inhibition against E. coli (27.0mm) and Pseudomonas aeruginosa (25.0mm) then methanolic, acetone and aqueous extracts. The Phytochemical analysis of Azadirachta indica and Withania somnifera was found to presence and absence of alkaloids, flavonoids, tannins, phenolic compounds, terpenoids, glycosides, steroids and Saponins. 11hese phytochemicals show the antimicrobial activity.