Dissertation on Investigating Mechanism of Neuronal Cell (N2A) Injury Induced by Shockwave

Abstract

The ohtccllvc of th1~ \tUd) 11 as to gam a comrrchrnm e unucr,tondmg of the tOt• I~ and techn1quc' u'cd m the 'tud) ot Neuro'C1cncc e'pc.iall) TBI In >~tro culture of 1\2<1 cells "a< pertormcd ond cell \UJ'\IVDI 11as as~esscd u<mg the f-11 I a"a)' alter subjec11ng the cells t<l 'hockwa\'C mtlll)'. Flm~ cy tomctry was utthlcd to ano lytc cellular en lcium lcvels Prntcu1s were extracted tmm N2A cell\ using cenlntugation. and thc1r quantlt} 11 as estimated U\lng the Bradford U~\~) Subsequent!) the protem• 11ere separated based on their molecular \\Cight usmg SDS-PAGI 1-unhermurc a compari:.on Ill protem bamh between the control and m1ur. -treated groups \\U\ performed usmg SDS-PAGr to identtf) an) damage induced h} !he sbnck \13\C In the in-s1hcu >IUd) ••f traumauc bram 1n1u~ ITBIJ rC)f'OMI\e proteins. tunaion.tl mtcracllon analysis 11 as conducted u\lng the String and Sutch datilha>C> The results 'hu11ed that shockwave treatment led to m~rea~cd neunte grow1h and decrea,ed cell number> m 1\~a celb. lhe \11T assa) re1caled a dcncavt m cellular metabolic actl\ltl \lith mcreasmg shockwave expo>ure doses. FkH• cytomclr} lmai)>IS demonstrated a ' 'llntficant increase 111 cytosolic calciUm content in shocl..waiC·C\JW'cd cells compared to control cells A standard cur\c for protein e<timation \\3S generated u~mg the Brodh>r<l JS<a). and SDS-I'AGF "as u~d to «parate and analpe protems ba,ed un m••lccular 11e1ght These lindmgs highlight the impact of 'huck"a'e mtUI') on cellular m<>rpholoS). mctJbol" actili~. calciUm le.els. and protein profiles. The otud) pr01idcs uluable in,ights mto the tO<•f, and techmquc' emplo) cd m neurob1oloiD re>~:<~rch 1nJ the.r appllc.1tion in 1n' e'l 'gatmg traumat1c h.am tnJUI) llndcr>tanding the molecular mcchon,.ms under!) mg. TBI cnn contnbutl· to the de~elopmcnt of etlcct•ve diagnost" and therapeUtic >tratcgies in the fu ture