Genetic Diversity Analysis in Tomato (Lycopersicon Esculentum) Using Rapd and Ssr Markers

Abstract

Genetic diversity of sixteen genotypes of tomato (Lycopersicon esculentum) using the SSR and RAPD markers. RAPD analysis with ten primers generated a total of 74 polymorphic bands. The number of amplification products produced by each primer varied from 2 to 6.Genetic diversity of sixteen genotypes in RAPD analysis as estimated by polymorphic information content (PIC) value ranged from 0.00 to 0.98. The cluster dendrogram of RAPD showed similarity values from 0.67 to 0.90.Dendrogram generated using RAPD data showed two major clusters. Cluster I consist of two genotypes however, cluster II included 14 genotypes. Dendrogram revealed that Pusa rubi was distinctly reiated Selection-7 at a similarity coefficient 0.74. SSR analysis generated a total of 120 bands with ten primers. The number of polymorphic allele ranged from 2 to 5 with different primers. Out of ten primers eight primers showed 100% polymorphism while, it was recorded 0% with two SSR primers. Genetic diversity using SSR data as estimated by PIC values ranged from G.61 to 0.97. The cluster dendrogram of SSR revealed 2 major clusters. Cluster I was the largest and included 13 genotypes while clusters II comprising of three genotypes. Dendrogram generated using SSR data showed that H-86 and Pusa gaurav show 90% similarity. Dendrogram of combined analysis revealed 2 major clusters. Cluster II was largest und included 14 genotypes while, clusters I comprising of two genotypes. It was observed ~hat RAPD markers can be successfully used to study the genetic variation in tomato as all the RAPD primers used in current studies showed 100% polymorphism. Keywords: RAPD, SSR, Marker, PIC, Genetic diversity