Effect of Cytokinin on Callus Induction, Shoot Multiplication and Auxin on Root Initiation in Gerbera (Gerbera Jamesonii Bolus)

Abstract

Present investigating was carried out on “Effect of growth hormones in induction of callus induction activity leaf explants in Gerbera Jamesonii (Bolus) in in vitro condition” at the Tissue culture Laboratory Department of Horticulture, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut during the year 2015-2019. All experiments were directed to the development of technology and motivated by practical demands. The aim of the work was to develop efficient callus inductions shoot multiplication as well as root initiation protocols in Gerbera. treatments showed that significantly minimum time of callus induction 29.76 days was observed in treatment 2,4-D @ 2.00 mgl-1+ Kinetin @ 1.50 mgl-1 with CHU media and showed that significantly minimum time of callus induction 31.85 days was observed in treatment 2,4-D @ 2.00 mgl-1+ Kinetin @ 1.50 mgl-1 with SH media. Significantly minimum time of callus induction 35.67 days was observed in treatment IBA @ 2.00 mgl-1+BAP @ 1.00 mgl-1 with CHU media and significantly minimum time of callus induction 37.89 days was observed in treatment IBA @ 2.00 mgl-1 +BAP @1.00 mgl-1 with SH media.