Microproipagation of Cfjloropf/ytum Borjvjljanull4 and Hts Clonal Fideilltv Through Rapd Lviarkers

Abstract

Chlorophytum borivilianum Sant. et. Fernand. Vern Safed musli, 'a golden root', a unique gift of nature to mankind since time immemorial as an alternative to chemical aphrodisiac. In the present investigation micropropagation was achieved through standardisation of shoot bud and stem base as the explants with different concentration of auxin and cytokinins. Shoot bud and stem base were cultured on Murashigae and Skoog (MS) media supplemented with concentration of BAP (range from 1-4 mg/1) and Kinetin (1-3mg/l). The better response was showed in MS medium containing 2 mg/1 BAP resulting more number of leaves, shoots and minimum days for shoot initiation. The high and intense response of root proliferation was observed on full strength of MS medium at second and fourth week respectively after inoculation with the increasing concentration (2 to 3 ,ng/1) for indole-3-butyric acid (IBA) and benzyl amino purine (BAP) at 2mg/l. The C.borivilianum plantlets survived better under artificial growth chamber condition as compare to direct exposure into the field. The true to type plants obtained were subjected for the somaclonal variation by checking its clonal fidelity through RAPD marker. Out of 11 primers three primers (OPA-01, OPA-06, and OPA-20) showed PCR amplification which reported negligible variation. All the bands were monomorphic resulting in 0.99 similarity coefficient and no polymorphism was found. Thus all the micropropagated plantlets were similar to the mother plants.