Studies on Morpho-physiological and Molecular Expression Analysis of Certain Transcription Factors Induced by Water Stress in Rice (Oryza Sativa L.)

Abstract

Water stress is a major factor limiting in rice production and affects global production and distribution of rice. Six rice varieties Pusa Basmati1, Pusa1121, Type3 and Vallabh basmati (Basmati type) and two non-basmati rice varieties Nagina 22 and Susk Samrat were selected for the water stress experiment. The study of water stress effect on morphological , biochemical and molecular expression showed wide range of variations among all the selected rice varieties. The result showed that all the morphological characteristics decreased under water stress condition. In overall performance the chlorophyll content, photosynthesis, relative water content, membrane stability index decreased when these plants varieties were subjected to water stress condition. Proline content increased in water stress tolerant rice variety during water stress condition. Gene expression analysis of various transcription factors AP37,AP59, OsMYB48-1,OsMYB-2, GeBP1, GeBP2 and genes OsLEA, OsP5CS2 related to water stress condition are up and down regulated under water stress condition compare to control condition