Studies on Development of Improved Production Technology for Milky Mushroom (Calocybe Indica)

Abstract

Ca/ocybe indica, one of the most important mushroom commonly called as "Kuduk" or "Dudhi chhata" or "Milky" mushroom. It was first reported from India by Purkayastha and Chandra in 1974. Five strains of Calocybe indica viz., Cl-3, Cl-4, Cl-5, Cl-6 and Cl-10 were observed for variability in terms of colony characters, cultural characters and yield performance. Fluffy growth appeared in the colony of strain Cl-3, Cl-4, Cl-5 and Cl-1 0. The colony colour of strains Cl-3 and Cl-1 0 were observed as creamy white. The average pileus breadth, stipe length and pileus weight was observed in strain Cl-6 (5.30 em, 4.37 cm&23.00 g/FB, respectively). At 28°C temperature and 7.5 pH strain Cl-6 showed maximum (9.0 em) growth of mycelium. Maximum growth was recorded in MEA medium (8.98 em) from strain Cl-4 and in ferrous sulphate (9.00 em), magenese sulphate (9.00 em) in Cl-6 strain. Full growth of strains Cl-6 and Cl-4 was found in barley grain+ gram husk (15.67 days) and wheat grain+gram husk (16.33 days) in spawn bottle. Maximum yield was recorded from two strains Cl-6 and Cl-4 with spawned wheat grain+gram husks (621 .67 and 609.67 g/kg of dry substrate with 62.17 and 60.97% B.E.). Maximum yield (613.00 and 603.00 g/kg of dry substrate with 61 .30 and 60.30% B.E.) at 6% spawn rate on wet weight basis were observed in strain Cl-6 and Cl-4, respectively. Maximum yield was harvested from the two strains Cl-6 (611 .33 g/kg of dry substrate with 61.13% B.E.) with WS+BS (2:1) and Cl-4 (536.33 g/kg of dry substrate with 53.63% B.E.) with WS+BS (1 :2). Maximum yield was harvested in soybean flour treatment from strains Cl-6 and Cl-4 (648.33 and 599.67 g/kg of dry substrate with 64.83% and 59.97% B. E.). Maximum yield was harvested with the use of magnesium sulphate treatment from strains Cl-6 and Cl-4 (559.00 and 552.33 g/kg of dry substrate with 55.90% and 55.23% B. E.). The soybean flour supplemented casing was significantly showed maximum yield (616.00 and 588.33 g/kg of dry substrate with 61 .60% and 58.83% B.E.) and from strain Cl-6 and Cl-4. Maximum yield was harvested in casing mixture FYM+VC+GS from strains Cl-6 and Cl-4 (607.00 and 591.67g/kg of dry substrate with 60.70% and 59.17% B.E.). Maximum yield was recorded in casing thickness 2.5 cm/1000gm from strains Cl-6 and Cl-4 (559.00 and 544.67g/kg of dry substrate with 55.90% and 54.47% B.E.). Minimum time was observed during June-August for spawn run (18.00 and 19.00 days). The maximum number of fruiting bodies was harvested during the month September-November in Cl-6 and Cl-4 strains (23.33 and 22.00/bag). While maximum yield was harvested during July-September from strains Cl-6 and Cl-4 (607.00 and 575.33 g/kg of dry substrate with 60.70% and 57.53% B.E.).