Identification, Characterization and gene expression of ZIP gene family in Glycine max

Abstract

Glycine max is an important hot summer crop which is the the most important plant source of vegetable oil and protein in the world. Zinc deficiency afflicts up to 40% of the world’s human population, mainly in developing countries where people depend on cereal-rich diets for sustenance, metals are transported from the soil into the root and then must cross both cellular and organelle membranes as they are distributed throughout the plant. Specific metal transporters are required. Such understanding will require knowledge of how metal levels are sensed by plants and how metals control gene expression. In the present study primers for metal transporter gene ZIP was designed by IDT tool. Basic nutrient nutrient were provided using Hoagland solution, cDNA libraries were prepared which resulted in ZIP gene of 364 bp with an accession number MN171530 characterized by its protein sequences, amino acid composition and also analyzed through phylogenetic tree and multiple sequence alignment. Expression analysis was done at different concentration and at different hours For 100 mM of zinc treatment, induction of ZIP gene was recorded at all time periods of zinc treatment; the highest level of gene expression was obtained at 6 hr whereas, lowest expression level was obtained for 24hrs of zinc treatment . For 150 mM of zinc treatment the transcription level of ZIP gene was highest at 12hr whereas, lowest expression level was obtained for 24hrs. For 200 mM of zinc treatment, the highest level of gene expression was attained for 6hr while expression gradually declined for 12hr and 24hr Overall ZIP gene expression were highest at 100 mM for 6hr (2fold) and lowest zinc treatment at 150mM for 24hr (0.65) therefore will help in divulging new sources of nutritionally important crop varieties by higher expression level of ZIP gene leading to improved staple food crops