Student Ready Project Formulation, Execution and Presentation (Bts-421) a Project Report On "Isolation and Identification of Escherichia Coli From Bovine Milk Sample in Meerut, Uttar Pradesh.

Abstract

A cross-sectional study was carried out from February to May 2024, to determine the presence of Escherichia coli in raw milk from dairy farms and farmer's cattle from nearby places of Meerut. A total of 15 samples were collected. 10 from dairy cattle’s and 5 from farmer's cattle. To proceed bacteriologically, the isolates were tested with a number of biochemical tests for confirmation and identification of E. coli. The study revealed that 40% of the collected raw milk was contaminated with E. coli. Besides, all E. coli isolates exhibited bright pink color with lactose fermentation on MacConkey agar plates, metallic sheen on Eosin Methylene Blue agar plate and gram-negative, pink-colored, small rod-shaped organisms arranged in single with pairs or short chains on Gram’s staining. In this study, there was significant (p< 0.05) association between the body condition, age, and breed of the cattle with the isolates of E. coli. In conclusion, the presence and consumption of raw milk may constitute a public health hazard and reduced milk quality due to E. coli. Thus, health professionals should create awareness about milk handling practice, storage and milking process to Dairy farmers and milk collectors.