Effect of Different Media, Grain Substrates and Additives on Performance of Oyster Mushroom (Pleurotus Florida, Pleurotus Flabellatus and Pleurotus Sapidus)

Abstract

Oyster mushrooms are the important and least expensive to grow, they are well known for conversion of crop residues to food protein (Banik and Nandi, 2004). The present investigations were carried out on “Effect of different media, grain substrates and additives on performance of oyster mushroom (Pleurotus florida, Pleurotus flabellatus and Pleurotus sapidus)” The results of present investigations showed that the colony colour of P. florida was creamy white and P. flabellatus was milky white, while P. sapidus was observed as shiny milky white in appearance. The circular colonies and even type of margin was observed in two species viz. P. florida and P. flabellatus but in case of P. sapidus circular colony and uneven type margin was observed. The species of P. sapidus gave maximum yield with attractive grayish fruiting bodies followed by P. florida was good yielder but produced more flashy, milky white and creamish white fruiting bodies, respectively while the P. flabellatus were low yielder and produced white fruiting bodies. Among the media, in Barley extract agar medium on 7th day full radial growth of mycelium (90.00 mm) was recorded in P. sapidus and P. Florida. Maximum weight of dried mycelium (6.01 mg/50ml) was observed in Oat extract broth medium while P. flabellatus maximum weight of dried mycelium (4.96 mg/50ml) was observed in Barley extract broth medium while In case of P. sapidus maximum weight of dried mycelium (5.07 mg/50ml) was observed in Barley extract broth medium while minimum weight of dried mycelial (3.07 mg/50ml) was observed in potato dextrose broth medium. For the spawn improvement maximum mycelial growth of strains P. florida, P. flabellatus and P. sapidus on maize and sorghum grains. P. sapidus maximum mycelium growth was observed in Maize grain (90 mm) and P. florida shows maximum mycelium growth was observed in Maize grain (89.25 mm) while minimum mycelium growth was found in Wheat grain substrate (54.5 mm) and in case of P. flabellatus maximum mycelium growth was observed in Maize grain (86.50 mm). Among the different inorganic additives (0.5%) added to the substrate, maximum yield (550.00 g/kg dry substrate) was observed in magnesium sulphate and number of fruiting body (81.95) was observed in Ammonium chloride in P. florida while in P. flabellatus maximum yield (487.50 g/kg dry substrate) and number of sporophores (69.58) was observed in Calcium nitrate and in case of P. sapidus maximum yield was observed (547.50 g/kg dry substrate) in Magnesium sulphate and number of sporophores (19.75) was observed in Ammonium chloride and Ammonium sulphate both.