Effect of Different Plant Growth Regulator on in-vitro Differentiation of Papaya

Abstract

Papaya is the most important fruit crop of tropical and sub-tropical countries of the world. It is valued for its nutritional as well as pharmaceutical importance as it is rich source of provitamin A, Calcium and Papain, a photolytic enzyme. Since Papaya is highly heterozygous fruit crop. There is need of multiplication of plant through Tissue Culture for bearing fruits in almost all the plants. Keeping these facts in view the multiplication of Papaya Carica papaya L. cv. Pusa Nanha through Tissue Culture is needed for the production of numerous plants with in short period of time that is why the present experiment was carried out in the Tissue Culture Lab of the Department of Ag. Biotechnology, Sardar Vallabhbhai University of Agriculture & Technology, Meerut, (U.P.) during the year 2020-2021. The experiment was conducted in two way factorial design with 22 treatments comparising of 3 level of BAP (1, 2 and 3 mg/L), 3 levels of Kinetin (0.5, 1 and 2 mg/L), 4 level of NAA(0.1, 0.5, 1, 2 and 3 mg/L) and 3 levels of TDZ(0.5, 1 and 1.5 µM) replicated thrice. The different concentrations of Auxins and Cytokinins alone and in various combination are applied. The effect of these combinations was clearly observed among the different concentration of the bio-regulators, higher percent of explants was produced callus in media containing Basal MS + 3.0 mg/L NAA + 0.5 mg/L Kin + 1.5 µM TDZ and this combination also have less time span for callus production. In case of shooting of explants MS Basal + BAP 1 mg/L + NAA 0.5 mg/L was found to be the best for shoot development and as well as for maximum number of shoot. hence on the basis of result obtained from the present investigation it can be concluded that the combination of Basal MS + 3.0 mg/L NAA + 0.5 mg/L Kin + 1.5 µM TDZ is the best for callus formation and MS Basal + BAP 1 mg/L + NAA 0.5 mg/L is the best for shoot development and maximum shooting respectively in MS media for Papaya (Carica papaya L.) tissue culture and can be recommended to the commercial tissue culture laboratories and to the researchers.