Molecular Diversity Analysis of Abelmoschus Esculentus

Abstract

Genetic Diversity Analysis of Abelmoschus escu/entus is important for its characterization. The genetic diversity of 22 genotypes of A. esculentus, 20 random primers were used of which 18 primers gave amplification and 15 resulted in polymorphic, scorable and reproducible results. On the basis of resolving power, primer NJ-5, 6 and 3 were most significant as they are able to recognize all 22 genotypes. The random primers used for estimating gene diversity were found most suitable for the purpose as they showed sufficient value. Cluster analysis clearly showed that some genotypes are closely related while some are significantly distinct. The study did not show the separation of individuals on the basis of their response towards YVMV infection. The protein profile of 22 genotypes of A. esculentus was also prepared and compared with RAPD data set. The present study suggests that RAPD and total protein profile among 22 genotypes of A. esculentus showed that both technique had excellent discriminating power and could accurately detect the structure of genetic diversity.