Studies on Spawn Quality of Shiitake Mushroom (Lentinula Edodes)

Abstract

Golden oak mushroom commonly known as Shiitake mushroom (Lentinula edodes), is the second most popular edible mushroom in the world which contributes about 26% in the production, it has yet to find a suitable market in India due to a lack of basic knowledge regarding cultivation, use and medical benefits. The present investigations were carried out on “Studies on spawn quality of shiitake mushroom (Lentinula edodes).” The result of present investigation on LE-22104 and LE-22106 of shiitake mushroom (Lentinula edodes) showed that circular colonies with raised type elevations, initially white in colour which later turned yellowish brown with radiating margins and form cottony white mat like appearance and fruiting bodies tan to brown coloured, characterized by whitish lamella and umbrella shaped with brown pileus and dense and subsequently greyish gills which was firmly stiped. In case of three different vitamins evaluated, maximum radial growth as well as maximum dry mycelium weight was observed in thiamine @50ppm in both the strains (LE-22104 and LE-22106). In case of three different trace elements analysed, maximum radial growth as well as maximum dry mycelium weight was observed in Manganese @3ppm in both the strains (LE-22104 and LE-22106). For spawn production, in case of pulse additives, maximum spawn growth of both strains (LE-22104 and LE-22106) was observed in Wheat + Pigeon pea flour @5%. In case of different carbon and nitrogen sources in combinations for spawn production, Wheat + Dextrose:Yeast extract @7:1 was found best for fast spwan growth in both starins (LE-22104 and LE-22106) . In case of different agro-residues on evaluated for sporophore production of strains (LE-22104 and LE-22106), maximum sporophore yield was observed in Saw dust.