Genome Wide Screening of Chickpea (Cicer Arietinum L.) for Drought Tolerant Gene Using Issr Marker

Abstract

Chickpea (Cicer arietinum L.) is an autogamous annual cool season grain legume cultivated in arid and semi-arid areas across the world including India. Drought is one of the most important abiotic stresses, which limits crop production in different parts of the country. Estimates of yield loss may be due to drought ranges from 15 to 60 percent depending on geographical regions, duration of the crop season and dry spell. Thirteen chickpea genotypes viz. BG226, Vijay, JAKI 9218, Pusa362, DCP92-3, JG16, K850, CSG8962, Avrodhi, KWRI08, RSG888, Pant 0114 and Phule 05 were evaluated for drought conditions and these genotypes exposed to drought at reproductive stage. Genotypes under drought were evaluated for different morphological, physiological, biochemical and molecular parameters. Genotypes CSG8962 and BG- 256 were found better under drought condition for the traits height, leaf area and proline content, as they have indigenous level of drought resistance. Level of molecular diversity observed among the genotypes under drought conditions using ISSR markers. Similarity coefficient was obtained in the form of dendogram for ISSR analysis. Jaccard's coefficient of similarity revealed that high degree of similarity to the extent of 86% exits between genotype Pant 0114, Phule G-5, K850, KWR108 where as JG-16, CSG 8962, RSG-888 and JAKI-9218 showed less similarity level of 60-63% to the member of other cluster. PIC value for ISSR ranges from 0.68 to 0.94. Out of ten ISSR markers, eight generated polymorphic bands. A total of 236 bands scored from ten primers of which 70% were polymorphic while three were monomorphic, the number of polymorphic alleles varied from 1 to 4. Hundred percent polymorphism were obtained from seven primers. Present study will give an insight for future research for isolation and characterization of drought responsible genes in chickpea.