Identificaiton and Molecular Characterizaiton of Phytoplasma Associated with Peach Disease in Western Uttar Pradesh

Abstract

Recently, peach trees showing symptoms of disease caused by phytoplasma like leaf reddening, yellowmg, leaf rolling symptoms were observed in peach grow1ng areas in western regions of Uttar Pradesh. In nature phytoplasma are transmitted by leafhopper that may have occasional or permanent trophic relationship with their host. Plant pathogenic phytoplasma are wall less, unculturable bacteria of class mollicates with a small genome size, which ranges from 530 to 1350 kilobases. In diseased plants, phytoplasma resides almost exclusively in the phloem s1eve tube elements. Different parts of peach tree such as infected bark and midrib from leaves were selected for the total DNA extraction with C-TAB method and w1th DNA isolation kit Quiagen (Germany) The Polymerase cha1n reactions (PCR) were performed us1ng Phytoplasma universal primer pairs. The PCR Product of -1800bp was obtained in the diseased plant. No PCR amplification was observed in the healthy plants. PCR product was cloned and sequenced. The Phylogenetic related to other Phytoplasma were evaluated by using iphyclassifier.