Longevity and Survival of Pseudomcnas Fluorescens at Different Moisture Level on Eem and Jatropha Cakes

Abstract

The present investigation were undertaken to test the suitability of neem and Jatropha cakes for mass multiplication and longevity of Pseudomonas jlourescens and Trichoderma harzianum in vitro. Jatrofa cake was found to be best substrate for supporting the population dynamics and longevity of P. jlourescens in vitro. Both these cakes supported the population of P. jlourescens up to 120 days. Highest population of P. jlourescens was noticed on jatrofa cake after 45 days of inoculation when maintained with I 5% moisture, while on neem cake highest population of P. jlourescens was noticed after 60 days of inoculation when maintained with 25% moisture. Jatrofa cake was better than neem cake for supporting population and longer shelf life of P. jlourescens in vitro. For T. harzianum, Neem cake was found to be best substrate for supporting the population dynamics and longevity as well in. vitro. Highest population of Trichoderma harzianum was noticed on neem cake with 25% moisture after 45 days of inoculation. Increasing in the moisture level resulted in enhancement of total viable count of T. harzianum on both cakes. Increasing concentration of extract (30%) of both cakes, resulted in increasing the population of P. jlourescens. Tomato plants grown in sterilized soil applied with P. fluorescens grown on Jatropha cake resulted in highest recovery of Pseudomonas flourescens from soil. Combined application oftwo bioagents in the rhizosphere oftomato in sterilized soil resulted in comparatively lower recovery of P. jluorescens and T. harzianum but was found better for root growth, shoot growth and fruit yield enhancement than their single application. Combined application of bioagents grown on any of the cake to the sterilized soil resulted in three time increase in root length, 1.5 times increase in shoot length and five time increase in fruit yields of tomato crop.