Isolation and Characterization of Native Rhizobium Strain From Black Gram(Vigna Mungo) and Its Efficiency as a Biofertilizer

Abstract

Strains of Rhizobium were isolated from Black gram (Vigna mungo) and the isolated strains were characterized biochemically including the detection of antibiotic sensitivity profile of the isolates and further these isolated Rhizobia! strains were utilised as a biofertilizer for Black gram variety cv. Urd Shekhar-2. Rhizobium was isolated from the root nodules of Black gram by standard microbiological methods and was confirmed by biochemical tests. The antibiotic susceptibility test was conducted by using the octadiscs, displaying commercially available antibiotics. After that these isolated strains were applied as a biofertilizer to the Black gram variety cv. Urd Shekhar-2 and several growth and yield parameters were recorded to evaluate the effect of Rhizobium on the selected variety and statistical analysis of these parameters were also carried out . NPK analysis of the Rhizobium treated soil was also done to estimate the biological nitrogen fixation done by the plant in presence of Rhizobium in its rhizosphere. The Rhizobia! strains isolated in our study showed the enhanced effects on the growth and yield parameters. Statistically also some of results recorded were found significant. NPK analysis also resulted in high nitrogen fixation by the plants in comparison to the control and fertilizer treated plants. The present study demonstrates that the inoculation of Rhizobia! strains to leguminous plants like Black gram resulted in an increase in the productivity and growth showing a positive effect of Rhizobium as a biofertilizer