Student Ready Entrepreneurial Development in Biotechnology 422 Development of an Onsite Detection Kit for Brucella Using Immunogenic/virulence Genes

Abstract

This abstract describ~s the development of an onsite antigen-based diagnostic kit for the early detection and treatment of infectious diseases, using in silica technology. In silica technology is a computer-based approach that involves the use of computational modelling and simulations to optimize the design of the diagnostic kit, the identification of the antigen, in silica design, physical prototyping, and testing. The development of onsite antigen-based diagnostic kits plays a crucial role in the rapid and accurate detection ofvarious infectious diseases. In recent years, Insilico technologies have emerged as powerful tools for accelerating the development process, reducing costs, and improving the efficiency of diagnostic kit design. Insilico technology, including computational modelling, molecular docking, and virtual screening, was employed to facilitate the rational design and optimization of the diagnostic kit. Through the use of Insilico techniques, the interactions between the target antigens and potential antibodies or receptors were predicted and evaluated. This enabled the identification of the most promising candidates for inclusion in the diagnostic kit. Furthermore, Insilico simulations aided in optimizing the assay conditions, such as reaction time, temperature, and concentration, to enhance the petformance and accuracy of the diagnostic kit. The Insilico driven development process significantly reduced the time and resources required for traditional experimental approaches. It allowed for a systematic evaluation of numerous antigen-antibody or antigenreceptor com in tion leading to the select" o~f optim.al pairs for the diagnostic kit