Morpho-molecular Characterization and Biochemical Evaluation of Okra (Abelmoschus Esculentus L.) Genotype

Abstract

The investigation entitled “Morpho-molecular characterization and biochemical evaluation of okra (Abelmoschus esculentus L.) genotype”. 15 okra genotypes were evaluated for molecular characterization and bio chemical evaluation and yield related traits with randomize block design (RBD) with three replication during kharif 2017. On morphological, molecular and biochemical evaluation on okra genotype. Observation were recorded on various characters viz., Days to flowering, Plant height (cm), Number of branches per plant, Length of fruit (cm), width of fruit (cm), Number of fruit per plant, Yield per plant (gm.) Maximum days to flowering was recorded in IC 90134. (48.7 days), highest plant height was registered for IC 34190. (117.20cm). Higher fruit length was recorded for IC 18544. (16.1cm), the maximum number of branches per plant (3.9) was recorded in IC 90242. The highest fruit width (2.2 cm) was recorded in IC18544. Maximum number of fruits per plant was recorded in IC 42456. (13.8). Whereas maximum fruit yield per plant was recorded in IC 33345 (111.4g). In the present study 10 RAPD primers were used by which a total of 15 polymorphic bands were obtained with an average of 2.8 bands per primer showing 100% polymorphism. Primer (OR-1) showed maximum value of PIC (0.87). Maximum RP showed by primer OR-9 (1.8). Cluster analysis based on untended paired group method of arithmetic means 15 okra genotypes were clustered into two main group like Group I and Group II, the minimum similarity exhibited by genotype IC 90096 and IC 90201 and the maximum similarity was shown by genotype IC 42456 with IC 42472. Among the studied varieties the proline content is present in significant amount in the leaf of okra varieties. The maximum proline content was in IC18546 genotype.